Confirmation of miRNAs differentially expressed between MS and PS using stem-loop real-time PCR.

<p>(A) miRNAs with expression levels up-regulated in PS compared with MS. (B) miRNAs down-regulated in PS compared with MS. (C) Northern blot analysis of the expression levels of zma-miR171b/f in MS and PS. Total RNA (20 µg) was loaded for each lane. U6 bands were used as a loading control. For (A) and (B), ordinates indicate relative expression levels.