Analysis of miRNAs by Northern blot hybridization and RT-PCR.

<p>Total RNA was hybridized with probes for known miRNAs (A) and putatively novel miRNAs (B) and RT-PCR with primers specific for putatively novel miRNAs (C) that are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042030#pone.0042030.s002" target="_blank">Table S2</a>. U6 in (A) and (B) was used as a loading control. Reactions without cDNA templates or primers were used as controls. Sizes of 100-bp DNA ladder (Bioline) are indicated.