miR-21 targets YOD1.

<p>(<b>A</b>) Wt and mut miR-21 targets within the 3′UTR of murine YOD1 mRNA. The target sites were predicted by using TargetScan program. Mutated sites were designed for luciferase assay controls. (<b>B</b>) miR-21 suppressed YOD1 expression. HL-1 cells were transfected and infected as indicated. YOD1 expression levels were measured by WB. (<b>C</b>) Luciferase assay to validate miR-21 targeting effect on YOD1 translation. HL-1 cells were co-transfected with miRNA mimics and luciferase reporters harboring wt or mut YOD1 3′UTR fragments. Dual luciferase assays were conducted to compare the relative luciferase activities (Firefly/Renilla) among different groups.