<p><b>A:</b> C2C12 cells transfected with luciferase constructs. MO25 3′ UTR target sequences (as indicated) of miR-195 (<b>left panel</b>) or miR-451 (<b>right panel</b>). Bar graph representation of luciferase expression in C2C12 cells treated with miR-195 (left panel) or miR-451 (<b>right panel</b>) mimics or negative siRNA control. Each bar indicates mean values ± S.E.M. of 3 separate experiments. *P<0.05 compared to WT group. <b>B:</b> Increase in expression of MO25 in C2C12 cell by miScript miRNA inhibitor delivered using HiPerfect reagent. AllStars Negative Control siRNA was used as negative control. Each bar indicates mean values ± S.E.M. of 3 separate experiments. *P<0.05 compared to WT group.</p
<p>(A) Schematic illustrating construction of the luciferase reporter constructs (MMP-2 UTR and MMP-...
<p>(A) The expression of Myc and the predicted miRNAs was validated by real-time PCR in the presence...
<p>(<b>a</b>) Putative miR-326 binding sequences in the 3′-UTR of the Bcl-xL and Bak genes and the m...
<p>Histograms showing relative expression (RQ) of mesenchymal markers (A), epithelial markers (B) an...
<p>(A) Cumulative distribution functions (cdfs) of log<sub>2</sub> fold change of mRNA expression be...
<p>(A) The miR-125b-5p responsive element in <i>MAD4</i> gene 3′UTR and the base-paired to miR-125b-...
<p>(A) The <i>Mbd1</i>-3′-UTR–dependent expression of Renilla luciferase reporter gene (R-Luc) was s...
<p>Panel A: WT reporter or mutated control luciferase plasmids were transfected into BON1 cells alon...
<p>(<b>a,b</b>) Luciferase assays using the CD24− and Src-3′-UTR or mutant reporter constructs trans...
<p>(A) Specific locations of the binding sites were marked with red color and MITF, BCL2 and cyclin ...
<p>(A) The sequences of miR-33b binding sites within the human c-Myc 3′UTRs and schematic reporter c...
<p>(A) Schematic diagram of miR-205 binding sites in the 3' UTR region of HMGB3 mRNA. (B) Luciferase...
<p>(A) The sequences of miR-410 binding sites within the human MDM2 3′UTRs and schematic reporter co...
<p>(A) <i>In silico</i> analysis, using TargetScan algorithms, showing a 8 mer seed match (grey box)...
(A). Luciferase activity in HEK293T cells cotransfected with miR-218-5p mimic (miR-218-5p; 10 nM) or...
<p>(A) Schematic illustrating construction of the luciferase reporter constructs (MMP-2 UTR and MMP-...
<p>(A) The expression of Myc and the predicted miRNAs was validated by real-time PCR in the presence...
<p>(<b>a</b>) Putative miR-326 binding sequences in the 3′-UTR of the Bcl-xL and Bak genes and the m...
<p>Histograms showing relative expression (RQ) of mesenchymal markers (A), epithelial markers (B) an...
<p>(A) Cumulative distribution functions (cdfs) of log<sub>2</sub> fold change of mRNA expression be...
<p>(A) The miR-125b-5p responsive element in <i>MAD4</i> gene 3′UTR and the base-paired to miR-125b-...
<p>(A) The <i>Mbd1</i>-3′-UTR–dependent expression of Renilla luciferase reporter gene (R-Luc) was s...
<p>Panel A: WT reporter or mutated control luciferase plasmids were transfected into BON1 cells alon...
<p>(<b>a,b</b>) Luciferase assays using the CD24− and Src-3′-UTR or mutant reporter constructs trans...
<p>(A) Specific locations of the binding sites were marked with red color and MITF, BCL2 and cyclin ...
<p>(A) The sequences of miR-33b binding sites within the human c-Myc 3′UTRs and schematic reporter c...
<p>(A) Schematic diagram of miR-205 binding sites in the 3' UTR region of HMGB3 mRNA. (B) Luciferase...
<p>(A) The sequences of miR-410 binding sites within the human MDM2 3′UTRs and schematic reporter co...
<p>(A) <i>In silico</i> analysis, using TargetScan algorithms, showing a 8 mer seed match (grey box)...
(A). Luciferase activity in HEK293T cells cotransfected with miR-218-5p mimic (miR-218-5p; 10 nM) or...
<p>(A) Schematic illustrating construction of the luciferase reporter constructs (MMP-2 UTR and MMP-...
<p>(A) The expression of Myc and the predicted miRNAs was validated by real-time PCR in the presence...
<p>(<b>a</b>) Putative miR-326 binding sequences in the 3′-UTR of the Bcl-xL and Bak genes and the m...