<i>miR-210</i> modulates the expression of multiple genes.

<p>Luciferase assays were performed 24 hours post-transfection on S2R+ cells transfected with miR-210 precursor (<i>pUAST-miR-210</i> + <i>p-Act-Gal4</i>) or negative controls (only <i>p-Act-Gal4</i>). (A) Luciferase relative levels in cells transfected with reporter constructs containing wild-type or mutant 3’-UTRs for the indicated genes, or a synthetic sequence including 3 perfect <i>miR-210</i> binding sites (sensor). Results from three independent experiments are shown as mean ± SD of firefly luciferase activity relative to controls. t test; *p<0.05; **p<0.01; ***p<0.005; ns, not significant. (B) Reporter constructs containing miR-210 wild type or mutant binding sites in <i>mnd</i>, <i>SoxN</i>, <i>Bsg</i>, <i>scrib</i> and 3’-UTRs (sensor). mut, mutation; del, deletion.