The 3′ UTR of AP-2α contains a MRE for miR-200b/200c/429 family.

<p>A) Diagram of luciferase reporter constructs. The predicted MRE, wild-type or MRE-deleted AP-2α 3′ UTRs were inserted downstream of the firefly luciferase gene of the pmirGLO vector. B) Repression of firefly luciferase by the interaction between miRNA and the predicted MRE. Each luciferase construct was co-transfected with miR-200b, miR-200c or miR-429 mimics into HEK293 cells. At 24 h post-transfection, the luciferase activity was examined. The firefly luciferase activity was normalized to Renilla luciferase activity. The firefly luciferase activity of the cells that were transfected with miRNA mimics was represented as the percent activity relative to that of the cells that were transfected with negative control miRNA mimics. Data are shown as the mean±SD of three independent experiments. **, p<0.01.