SOD1 was a direct target of miR-206.

<p>(A) Putative miR-206-binding sequences in the 3’UTR of SOD1 mRNA, identified by RNAhybrid 2.2. (B) The predicted miR-206-SOD1 binding structure, identified by RNAhybrid 2.2. (C) Schematic diagram of constructed vectors. The 3’UTR region of SOD1 was cloned downstream of the luciferase reporter gene (pGL3-promoter vector). (D) The sequence of the luciferase vector in pGL3-SOD1-UTR-WT and pGL3-SOD1-UTR-Del. (E) Primary canine myocardial cells were transfected with <i>Firefly</i> luciferase expression vectors with pGL3-SOD1-UTR. Luciferase activity was assayed 24 h after transfection. The <i>Firefly</i> luciferase activity of each sample was normalized to <i>Renilla</i> luciferase activity. The normalized luciferase activity of the cells transfected with the control lentivirus was set as 100% relative luciferase activity. The column graphs show the means of at least three independent experiments performed in duplicate. (F) Tissues of the LSFPs were collected in each group. SOD1, and internal standard GAPDH protein levels were detected as above. Western blot bands were normalized to GAPDH. **<i>P</i> < 0.01.