Analysis of tau signal with non-commercial monoclonal antibodies by Western blotting.

<p>The proteins were extracted form the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice with n = 3 for each group. Proteins were extracted with SDS-PAGE and then identified with the following commercial monoclonal antibodies: A: MC1, B: MC6, C: TG3, D: TG5, E: CP13, F: CP27, G: PHF-1, H: DA9. Normal anti-mouse (1) and Mouse TrueBlot ULTRA (2) secondary antibodies were used to detect primary antibodies. The heat stable fraction was used to remove non-specificity (3). Quantifications of the blots are available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094251#pone.0094251.s002" target="_blank">Figure S2</a>.