Analysis of tau signal with polyclonal antibodies by Western blotting.

<p>Proteins were extracted from the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg-AD mice. Proteins were separated by SDS-PAGE and then identified with the following polyclonal antibodies: A: Total Tau, B: pS199, C: pS396, D: pS404, E: pT205, F: pS422, G: pS262 and H: pS409. Normal anti-rabbit secondary antibodies were used to detect primary antibodies. The heat stable fraction was used to remove non-specificity: I: pS262 and J: pS409. Quantifications of the blots are available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094251#pone.0094251.s005" target="_blank">Figure S5</a>.