Analysis of tau signal with anti-mouse light chain secondary antibodies by Western blotting.

<p>The proteins were extracted form the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice with n = 3 for each group. Proteins were extracted with SDS-PAGE and then identified with the following commercial monoclonal antibodies: A: AT8, B: AT270, C: Tau46 and D: GAPDH. We used one antibodies of each non-specificity category (AT8 for high non-specificity, AT270 for moderate non-specificity and Tau46 for absence of non-specificity). Anti-mouse light chain secondary antibodies were used to detect primary antibodies except for GAPDH. Quantifications of the blots are available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094251#pone.0094251.s004" target="_blank">Figure S4</a>.