Antibody MHT2 specifically recognizes tau in western blots of tau-transfected HEK cells and mouse brain homogenates.

(A) Immunoblot of gels loaded with duplicates of tau-transfected HEK293T cell (HEK-tau) extracts as well as non-transfected cell (HEK) extract and a single lane of 200 ng recombinant 2N4R human tau (2N4R tau). The immunoblot was sequentially probed with tau antibodies MHT2 and 3026, and a GAPDH antibody. (B) Immunoblot of a gel loaded with soluble fractions of fractionated brain homogenate. 6 month-old, female tissue from a nontransgenic mouse (NT), a tau knockout mouse (Tau-KO), and an rTg4510 mouse are loaded in duplicate. This immunoblot was probed with antibodies MHT2, 3026, and GAPDH. (C) Immunoblot of a gel loaded with sarkosyl detergent insoluble fractions from the same samples shown in B. In this case the immunoblot was only probed with MHT2 and 3026, as GAPDH is undetectable in the detergent-insoluble fraction. The mobility of molecular mass markers is indicated on the left of each blot.