Effect of the preclearing on tau signal by Western blotting.

<p>The proteins were extracted form the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice with n = 3 for each group. The supernatant obtained from tissue homogenization was processed either in the normal manner to obtain total (non-cleared) sample (1) or processed to prepare cleared samples with Dynabeads (2) or protein G (3). Protein were extracted with SDS-PAGE and then identified with the following antibodies for both total and cleared samples: A: AT8, B: AT180, C: PHF-1, D: DA9, E: total tau, F: GAPDH. Quantifications of the blots are available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094251#pone.0094251.s003" target="_blank">Figure S3</a>.