Whole kidney gene expression (mRNA) as measured by quantitative RT/PCR (qRT-PCR) for wild type and HYP mice infused with vehicle or SPR4-peptide (see also <b>Figure 3</b>).

<p>Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice, SPR4 = infused SPR4-peptide and Vehicle = Saline infused. For gene analysis mice were sacrificed on day 28 and mRNA prepared from whole kidneys as detailed in methods (N = 6). For qRT-PCR gene analysis fold differences in expression calculated by the Pfaffl method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097326#pone.0097326-Pfaffl1" target="_blank">[163]</a> were statistically analyzed for significance using the One Sample t-test and the Wilcoxon Signed rank-test with theoretical means set to 1. Results for all tests were considered to be significantly different at p<0.05 and are denoted with an asterisk (*) superscript. The fold changes in expression and standard error of the means (SEM) of <i><u>WT-VE versus HYP-VE</u></i>, <i><u>WT-VE versus WT-SPR4</u></i> and <i><u>HYP-VE versus HYP-SPR4</u></i> are shown in columns 1, 2 and 3 as indicated in the headings. ND = Not done, NS = Not Significant. <b><i><u>Index</u></i></b>: <b>NPT2a</b> = Sodium-dependent phosphate co-transporter (Slc34a1); <b>NPT2c</b> = Sodium-dependent phosphate co-transporter (Slc34a3); <b>1-α-Hydroxylase</b> = 25-hydroxyvitamin D₃ 1-alpha-hydroxylase (CYP27B1); <b>24-Hydroxylase</b> = 1,25-hydroxyvitamin D₃ 24-hydroxylase (CYP24A1); <b>VEGF</b> = Vascular Endothelial Growth factor; <b>SOST</b> = Sclerostin; <b>GAPDH</b> = Glyceraldehyde 3-phosphate dehydrogenase; <b>Cyclophilin</b> = peptidylprolyl isomerase A (cyclophilin A).