RT-PCR analysis of transcripts of interest.

<p>Quantitative RT-PCR was performed to investigate if proteins of interest showing a ≥2 fold change at the protein level were also regulated at the mRNA transcript level between the Lp(a) and wildtype mice. Total RNA was isolated from aorta samples (n = 6) of wildtype and Lp(a) mice. RNA was reverse transcribed to cDNA and quantitative PCR for transcripts of interest relative to 18S rRNA was performed. Glucose-6-phosphate dehydrogenase (G6pdx) and peroxiredoxin 4 (Prdx4) transcripts were increased in Lp(a) mice compared to wildtype. Dihydrolipopoyllysine succinyltransferase (Dlst), Glycerol-3-phosphate dehydrogenase (Gpd1) and fatty acid-binding protein 4 (Fabp4) transcripts were decreased in Lp(a) mice compared to wildtype. Isocitrate dehydrogenase (Idh3a) transcript showed no significant difference in Lp(a) mice compared to wildtype mice. Results are presented as relative levels of transcript normalized to wildtype. ^***<i>P</i><0.001 versus wildtype.