Bone (femur) gene expression (mRNA) as measured by quantitative RT/PCR (qRT-PCR) for wild type and HYP mice infused with vehicle or SPR4-peptide (see also <b>Figure 4</b>).

<p>Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice, SPR4 = infused SPR4-peptide and Vehicle = Saline infused. For gene analysis mice were sacrificed on day 28 and mRNA prepared from bone marrow stromal cell depleted femurs as detailed in methods (N = 6). For qRT-PCR gene analysis fold differences in expression calculated by the Pfaffl method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097326#pone.0097326-Pfaffl1" target="_blank">[163]</a> were statistically analyzed for significance using the One Sample t-test and the Wilcoxon Signed rank-test with theoretical means set to 1. Results for all tests were considered to be significantly different at p<0.05 and are denoted with an asterisk (*) superscript. The fold changes in expression and standard error of the means (SEM) of <i><u>WT-VE versus HYP-VE</u></i>, <i><u>WT-VE versus WT-SPR4</u></i> and <i><u>HYP-VE versus HYP-SPR4</u></i> are shown in columns 1, 2 and 3 as indicated in the headings. ND = Not done, NS = Not Significant. <b><i><u>Index</u></i></b><u>:</u><b>FGF23</b> = Fibroblast Growth Factor 23; <b>MEPE</b> = Matrix Extracellular Phosphoglycoprotein with ASARM -motif; <b>DMP1</b> = Dentin Matrix Protein 1; <b>SOST</b> = Sclerostin; <b>VEGF</b> = Vascular Endothelial Growth factor; <b>GAPDH</b> = Glyceraldehyde 3-phosphate dehydrogenase; <b>PHEX</b> = Phosphate-regulating gene with Homologies to Endopeptidases on the X chromosome; <b>Osteocalcin</b> = Bone gamma-carboxyglutamate (gla) protein; <b>Cyclophilin</b> = peptidylprolyl isomerase A (cyclophilin A); <b>Plin-2</b> = Perlipin-2; <b>ESP</b> = Osteotesticular protein tyrosine phosphatase (OST-PTP); <b>FAM20C</b> = Family with sequence similarity 20, member C Kinase also known as DMP4; <b>ENPP1</b> = Ectonucleotide Pyrophosphatase Phosphodiesterase 1.