Real-time Quantitiative PCR analyses on kidney tissue from <i>Fnip1</i>^<i>-/-</i> versus wildtype mice.

<p>RNA was purified from <i>Fnip1</i>^<i>-/-</i> (n = 3) compared to WT (n = 3) kidney cortical tissue (mice were 11–18 weeks of age). Quantitative PCR was performed on cDNA derived from these tissues. Bar graphs show fold- differences in gene expression in <i>Fnip1</i>^<i>-/-</i> compared to WT mice (where WT is set to 1). (A) Genes encoding many of the solute carrier (Slc) family of transporter proteins are decreased in <i>Fnip1</i>^<i>-/-</i> compared to WT kidney tissue. (B) Altered expression of genes encoding acyl-CoA regulatory molecules and fatty acids regulatory molecules. (C) Expression of S100A genes including A14, 6, 8, and 9 are increased in <i>Fnip1</i>^<i>-/-</i> compared to WT kidney tissue. (D) Increased expression of TLR1 and 13 genes in <i>Fnip1</i>^<i>-/-</i> compared to WT kidney tissue. (n = 3 of each genotype, * = p<0.01).