CDAA diet caused hepatic steatosis in <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice.

<p>A. Histology of the livers of <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice fed the CSAA (left) or CDAA (right) diets. Representative changes of the liver with regard to fat deposition at 4 (upper), 12 (middle), and 20 (lower) weeks during the experiments are depicted. Fat deposits were confirmed by oil red O staining shows (orange, inset). CV indicates central vein, and PT marks portal triad. Bars indicate 100 µm. B. Quantification of triglyceride in the liver. Triglyceride in the liver was increased in the livers of both <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice during administration of the CDAA or CSAA diets, although it was significantly more prominent in <i>Nrd1^+/+</i> mice. n = 4–5, each. *<i>P</i><0.05. C. There was no significant difference in the liver/body weight ratio between <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice during the experiments.