Hepatic steatosis.

<p>In mice fed the LF-NP and HF-NP large amounts of lipid droplets can be detected after 1 week and 12 weeks of dietary intervention. Mice fed the high protein diets (LF-HP and HF-HP) these lipid droplets are almost absent. (A) Oil red O staining of representative 5 µm liver sections of the 8 groups. Pictures were taken with a light microscope at 20x magnification. Bar represents 50 µm. (B) Hepatic triglycerides (mean expressed in mg/g liver) are decreased by HP feeding after 1 and 12 weeks and increased by HF feeding after 12 weeks. Effects of increased dietary protein (P) and fat (F) within one time point were tested using general linear model with P and F as fixed factors. * represents values significantly different from the reference group (ref.) as determined by one way ANOVA: *p≤0.05, **p≤0.01, ***p≤0.001.