Liver fibrotic area was not observed in <i>Nrd1^−/−</i> mice fed the CDAA diet.

<p>A. Liver fibrosis was determined by Sirius red staining (red) in <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice at 4 (upper), 12 (middle), and 20 (lower) weeks in the livers of <i>Nrd1^+/+</i> and <i>Nrd1^−/−</i> mice fed the CSAA or CDAA diet. Fibrotic changes were not observed in <i>Nrd1^+/+</i> or <i>Nrd1^−/−</i> mice fed the CSAA diet (left). Fibrotic changes were prominent in <i>Nrd1^+/+</i> mice, but not in <i>Nrd1^−/−</i> mice fed the CDAA diet (right). Bars indicate 100 µm. B. Quantification of fibrotic areas. Fibrotic areas was observed and increased in a time-dependent manner only in the livers of <i>Nrd1^+/+</i> mice fed the CDAA diet. n = 5–8, each. *<i>P</i><0.05.