Time-lapse of vasculature-like structure formation.

<p>Time-lapse images revealed the gradual development of a neurovascular cytoarchitecture in hCMEC/hNSC coculture. Arrowheads indicate the border of VLS that became prominent after coculture for 72 h. This solid cytoarchitecture was fixed after an observation period of 168 h. The contrast at borders between hCMECs (CD31+ in red) and hNSCs (polyclonal GFAP+ in green) areas was enhanced computationally. Diamidino-2-phenylindole (DAPI, blue) serves as a nuclear counterstain. Scale bar, 200 µm.