Depletion of <i>CYC9</i> in the bloodstream form inhibits cytokinesis.

<p>A. DAPI staining of nuclei (N) and kinetoplasts (K) at the time points indicated (post-induction). >200 cells per time point were classified according to their N/K configuration. B. Cytokinesis stage analysis of 2N2K cells. 2N2K cells (<i>n</i> > 200/timepoint) were scored by differential interference contrast (DIC) microscopy for whether or not they had a visible cleavage furrow (furrow ingression) or were undergoing abscission. Error bars represent the standard deviations from three replicate experiments. C. Flow cytometry analysis of propidium iodide stained cells at the time points indicated (in hours). The ploidies of the peaks are indicated. D. Example images of multinucleate/kinetoplast cells. Left panels: DIC image; right panels: DAPI staining. The N/K configuration of each cell is indicated. Scale bars: 5 µm. Arrows indicate partially ingressed cleavage furrows.