Quantification of endothelial morphogenesis.

<p>(A) A distinctive neurovascular cytoarchitecture emerged in which hCMECs (CD31+) formed vasculature-like structures (VLS) resembling a vascular network in between patches of hNSCs (polyclonal GFAP+). (B) The efficiency of VLS formation was quantified by measuring the length of segments between VLS branching points. (C) In a few samples, singular capillary-like structures comprising single layers of CD31+ cells between which a lumen-like space formation was observed. Diamidino-2-phenylindole (DAPI, blue) serves as a nuclear counterstain. Scale bars represent 50 µm.