Morphological pictures of HUVEC/HUASMC co-cultures upon physiological FSS conditions.

<p>(<b>A</b>) Scanning electron microscopy picture of the homogenously colonized inside of a hollow fiber with confluently grown and characteristically cobblestone shaped human primary endothelial cells upon the application of low laminar FSS (0.1 N/m²) for 24 h (magnification: 1∶500). (<b>B</b>) Scanning electron microscopy picture of HUASMCs on the hollow fiber outside with their typical cell cytoskeletal structure and morphology upon 0.1 N/m² applied for 24 h (magnification: 1∶400). (<b>C</b>) Confocal microscopic immunolocalization of Cadherin-5 in co-cultivated HUVECs exposed to low laminar FSS (0.1 N/m²) over a period of five days (magnification: 1∶400). (<b>D</b>)Cadherin-5 in HUVECs upon high laminar FSS (3 N/m²) (magnification: 1∶400). (<b>E</b>) Confocal microscopic immunolocalization of α-smooth-muscle-actin in co-cultivated HUASMCs upon 3 N/m² luminally applied for a five day period (magnification: 1∶400). (<b>F</b>) α-smooth-muscle-actin in co-cultivated HUASMCs upon high laminar FSS (3 N/m²) (magnification: 1∶400).