Charts displaying the <i>in vivo</i> radial positioning and expression profile of <i>B. glabrata</i> actin gene in the interphase nuclei of cells derived from NMRI (A) and BS-90 (B) snail strains, pre and post exposure to <i>S. mansoni</i> to normal miracidia (top row of charts, in red) or irradiated attenuated miracidia (bottom row of charts, in grey) over 30 minutes, 2 hours, 5 hours and 24 hours.

<p><i>B. glabrata</i> snails were infected with miracidia, dissected, fixed, and subjected to 2-D FISH or RNA was isolated and q-RT-PCR was performed (middle chart). In the NMRI snail strain <i>actin</i> is repositioned within interphase nuclei after infection and this is correlated with changes in gene expression (A). No repositioning is observed in the BS-90 snails, however the gene is expressed 30 minutes after infection (B). No repositioning of gene loci or change in expression was observed for <i>actin</i> in snails infected with attenuated miracidia. Statistically significant differences, as assessed by two-tailed Student's t-test, between normalized gene signal in each shell of control nuclei compared with infected snail nuclei are indicated by a black asterisk (P<0.05). Student's t-test between normalized gene signal in each shell of NMRI snail nuclei compared with BS90 snail nuclei are indicated by a red asterisk (P<0.05). Error bars = S.E.M.