Biomphalysin mRNA tissue distribution and expression in <i>B. glabrata</i> in response to <i>S. mansoni</i> challenge and in Bge cells in contact with sporocysts.

<p>A. Different <i>B. glabrata</i> tissues were analyzed by PCR using primers recognizing full-length Biomphalysin; actin was amplified as an endogenous control. Non-reverse transcribed hemocyte RNA was used as a negative control in PCR. B. Biomphalysin transcripts were quantified by Q-PCR in <i>B. glabrata</i> challenged with <i>S. mansoni</i> for 3, 6, 9, 12, 24, 48 and 96 h and in Bge cells in contact with sporocysts for the same intervals. Biomphalysin mRNA level was normalized to mRNA ribosomal protein S19 transcript abundance using the Roche Applied Science E-method <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003216#ppat.1003216-Tellmann1" target="_blank">[86]</a>. For graphical representation, the transcription ratio for each challenge was normalized to that obtained for unchallenged snails. Each histogram represents the average value of triplicate experiments ± SD.