<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells were acquired followed by gating on size versus granularity followed by exclusion of dead cells and finally detection of markers described in plots.</p
<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-a...
<p>(A) The gate was set using sizing microparticles (<1.5mm). Endothelial apoptotic microparticles w...
<p>A) & C) SSC vs FSC plots from 2D cultures and cells isolated from CM3D cultures, respectively. Ga...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>A: Gate used to exclude cell fragments and debris. B: Gate made to identify CD45- cells. C: CD31 ...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>A) An FSC/SSC gate and anti-CD45<sup>+</sup>/SSC was created around the viable lymphocyte populat...
<p>A, Stepwise selection for Sca-1<sup>+</sup>, CD31<sup>+</sup>, CD34<sup>dim</sup> and CD45<sup>− ...
<p>The triple positive phenotype (CD34<sup>+</sup>/KDR<sup>+</sup>/CD133<sup>+</sup>) was determined...
<p>CD45<sup>+</sup> cells were gated and proportions of CD14<sup>+</sup>, CD3<sup>+</sup>, CD15<sup>...
<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-a...
<p>(A) The gate was set using sizing microparticles (<1.5mm). Endothelial apoptotic microparticles w...
<p>A) & C) SSC vs FSC plots from 2D cultures and cells isolated from CM3D cultures, respectively. Ga...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>A: Gate used to exclude cell fragments and debris. B: Gate made to identify CD45- cells. C: CD31 ...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>A) An FSC/SSC gate and anti-CD45<sup>+</sup>/SSC was created around the viable lymphocyte populat...
<p>A, Stepwise selection for Sca-1<sup>+</sup>, CD31<sup>+</sup>, CD34<sup>dim</sup> and CD45<sup>− ...
<p>The triple positive phenotype (CD34<sup>+</sup>/KDR<sup>+</sup>/CD133<sup>+</sup>) was determined...
<p>CD45<sup>+</sup> cells were gated and proportions of CD14<sup>+</sup>, CD3<sup>+</sup>, CD15<sup>...
<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-a...
<p>(A) The gate was set using sizing microparticles (<1.5mm). Endothelial apoptotic microparticles w...
<p>A) & C) SSC vs FSC plots from 2D cultures and cells isolated from CM3D cultures, respectively. Ga...