<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells were acquired followed by gating on size versus granularity followed by exclusion of dead cells and finally detection of markers described in plots.</p
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD...
<p>Representative example of monocyte subsets depicted as classical CD14++CD16- monocytes (blue colo...
<p>–<b>C)</b> Flow cytometry gating strategy used to calculate the monocyte subpopulation bead-label...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>Dead cells were excluded by selecting all live cells from the forward/side scatter plot (A), afte...
<p>Mon1 indicates CD14++CD16− monocytes; Mon2 indicates CD14++CD16+ monocytes; Mon3 indicates CD14+C...
<p>(<b>A</b>) Percentage of the different circulating monocyte subpopulations, with a representative...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>PBMC were stained with mouse monoclonal antibodies and then analyzed by flow cytometry. Monocytes...
<p>Peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) were obtai...
Monocytes are key contributors in various inflammatory disorders and alterations to these cells, inc...
<p>Dead cells and red blood cells were excluded by selecting all live cells from the forward/side sc...
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD...
<p>Representative example of monocyte subsets depicted as classical CD14++CD16- monocytes (blue colo...
<p>–<b>C)</b> Flow cytometry gating strategy used to calculate the monocyte subpopulation bead-label...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>Dead cells were excluded by selecting all live cells from the forward/side scatter plot (A), afte...
<p>Mon1 indicates CD14++CD16− monocytes; Mon2 indicates CD14++CD16+ monocytes; Mon3 indicates CD14+C...
<p>(<b>A</b>) Percentage of the different circulating monocyte subpopulations, with a representative...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>PBMC were stained with mouse monoclonal antibodies and then analyzed by flow cytometry. Monocytes...
<p>Peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) were obtai...
Monocytes are key contributors in various inflammatory disorders and alterations to these cells, inc...
<p>Dead cells and red blood cells were excluded by selecting all live cells from the forward/side sc...
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD...
<p>Representative example of monocyte subsets depicted as classical CD14++CD16- monocytes (blue colo...
<p>–<b>C)</b> Flow cytometry gating strategy used to calculate the monocyte subpopulation bead-label...