Circulating MPA and monocyte characterization.

<p>(<b>A</b>) Percentage of the different circulating monocyte subpopulations, with a representative whole blood flow cytometry dot plot showing CD14 and CD16 expression, as well as accumulated data from n = 10 experiments presented graphically. (<b>B</b>) Representative flow cytometry dot plots for CD14 and CD11b (left) or CD11c (right) in the different monocyte subsets. Graph displays MFI values for CD11b (filled bars) and CD11c (open bars) in each subset (n = 10). (<b>C</b>), Representative flow cytometry dot plots for CD14 and TLR-2 (left) or TLR-4 (right) in the different monocyte subsets. Graph displays MFI values for TLR-2 (filled bars) and TLR-4 (open bars) in each subset (n = 10). (<b>D</b>) Representative flow cytometry dot plots for CD14 and CD42b (left panel) or CD62P (right panel). Graph shows data accumulated from n = 10 experiments illustrating the % MPA formed by the different monocytic subsets, as determined from CD14⁺CD42b⁺ (filled bars) or CD14⁺CD62P⁺ events (open bars). In black, red and blue are CD14⁺CD16⁻ (R1), CD14^highCD16⁺ (R2) and CD14^lowCD16⁺ (R3). *,**, *** p<0.05, <0.01 and <0.001 respectively <i>vs</i> R3. ^{†, ‡} p<0.05 and <0.01 respectively <i>vs</i> R1.