Flow cytometric gating strategy for monocyte subsets in rat peripheral blood.

<p>Dead cells and red blood cells were excluded by selecting all live cells from the forward/side scatter plot (A), after which a wide gate around the monocytes and granulocytes was selected (B) and copied to a CD172a/CD43 plot (C). The total monocyte population (used for calculating the total monocyte numbers) was selected from the CD172a/CD43 plot (C) and subsequently plotted in a new graph to calculate the percentages of the two classical (CD43-lo) and nonclassical (CD43-hi) subsets in the total monocyte population (D).