Gating strategy used for monocyte subset discrimination.
- Konstantin A. Krychtiuk (585228)
- Stefan P. Kastl (718059)
- Stefan Pfaffenberger (718060)
- Max Lenz (718061)
- Sebastian L. Hofbauer (718062)
- Anna Wonnerth (585229)
- Lorenz Koller (718063)
- Katharina M. Katsaros (585226)
- Thomas Pongratz (215348)
- Georg Goliasch (718064)
- Alexander Niessner (718065)
- Ludovit Gaspar (718066)
- Kurt Huber (585231)
- Gerald Maurer (602538)
- Elisabeth Dostal (718067)
- Johann Wojta (585232)
- Stanislav Oravec (718068)
- Walter S. Speidl (585220)
Publication date
April 2015
DOIAbstract
<p>Monocytes were defined as CD45 positive cells (B) exhibiting a typical forward (FSC) and sideward scatter (SSC) profile (A). To exclude possible contamination with T-cells, B-cells and Natural Killer cells, cells that stained for CD3, CD19 and CD56 were excluded, respectively (C). Remaining CD45+CD3/19/56- cells with a typical FSC/SSC profile were considered monocytes and distinguished according to their CD14 and CD16 surface expression into classical monocytes (CD14++CD16-), intermediate monocytes (CD14++CD16+) and non-classical monocytes (CD14+CD16++) (D).</p
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