<p>FSc-SSc profile was used to distinguish lymphocytes, monocytes and granulocytes (A). Within the lymphocyte population, B lymphocytes, T lymphocytes, and NK cells were identified using membrane markers CD19, CD3 and CD16/CD56, respectively (B, D). Monocytes were either CD14<sup>+</sup>/CD16<sup>low</sup> or CD14<sup>low</sup>/CD16<sup>+</sup> (C).</p
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
<p>The leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in...
<p>(A) Monocytes (CD14<sup>+</sup>), B cells (CD19<sup>+</sup>) were gated from PBMC. pDC were direc...
<p>Monocytes were defined as CD45 positive cells (B) exhibiting a typical forward (FSC) and sideward...
<p>Monocytes were first selected on the basis of their physical parameters, i.e. forward scatter (FS...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p>The lymphocytes were identified as R1 based on their size and granularity flow cytometric feature...
<p>The different leukocyte subtypes were analyzed by the shown gating strategy. In all CD45 positive...
<p><b>A.</b> PBMC and <b>B.</b> MBC. Initial gating is performed from the SSC-A vs. FSC-A to determi...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>Gating Strategy. Panel A. Identification of total monocytes from peripheral blood mononuclear cel...
<p>Whole blood monocytes were identified by a forward scatter and side scatter plot (A). Lymphocytes...
The % of CD3 (CD3+CD19-), the % of CD4 (CD3+CD4+), the % of CD8 (CD3+CD8+) and the % of CD19 (CD3-CD...
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD...
<p>G: Granulocytes, M: Monocytes, L: Lymphocytes, FSC: Forward scatter, SSC: Side scatter.</p
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
<p>The leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in...
<p>(A) Monocytes (CD14<sup>+</sup>), B cells (CD19<sup>+</sup>) were gated from PBMC. pDC were direc...
<p>Monocytes were defined as CD45 positive cells (B) exhibiting a typical forward (FSC) and sideward...
<p>Monocytes were first selected on the basis of their physical parameters, i.e. forward scatter (FS...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p>The lymphocytes were identified as R1 based on their size and granularity flow cytometric feature...
<p>The different leukocyte subtypes were analyzed by the shown gating strategy. In all CD45 positive...
<p><b>A.</b> PBMC and <b>B.</b> MBC. Initial gating is performed from the SSC-A vs. FSC-A to determi...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>Gating Strategy. Panel A. Identification of total monocytes from peripheral blood mononuclear cel...
<p>Whole blood monocytes were identified by a forward scatter and side scatter plot (A). Lymphocytes...
The % of CD3 (CD3+CD19-), the % of CD4 (CD3+CD4+), the % of CD8 (CD3+CD8+) and the % of CD19 (CD3-CD...
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD...
<p>G: Granulocytes, M: Monocytes, L: Lymphocytes, FSC: Forward scatter, SSC: Side scatter.</p
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
<p>The leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in...
<p>(A) Monocytes (CD14<sup>+</sup>), B cells (CD19<sup>+</sup>) were gated from PBMC. pDC were direc...