Gating strategy of bovine monocyte subsets based on relative CD14 and CD16 expression.

<p>(<b>A</b>) Three-color immunofluorescence of bovine MNC with mAbs to CD172a, CD14 and CD16 defines three monocyte subsets in peripheral blood. Viable (propidium-iodide-negative) mononuclear cells, based on forward and side scatter characteristics, were gated on CD172a-positive cells. Dot plots of CD14 and CD16 expression display classical monocytes (CD14+CD16−, upper left), intermediate monocytes (CD14+CD16+, upper right) and nonclassical monocytes (CD14-CD16+, lower right). (<b>B</b>) Monocytes were gated in a side scatter/CD172a dot plot, identifying monocytes as CD172a-positive cells. Histograms show that CD172a-positive cells do not express common markers of B cell (CD21), T cell (CD2, CD4 or CD8), NK cell (NKp46) or blood γδ T cell (WC1). Data are shown for cells from one of five tested animals.