<p>Lymphocytes and monocytes were gated based on forward and side scatter characteristics. B cells were CD3-CD4-CD8-, NK cells were CD3-CD8+, and monocytes were defined as CD3-CD4<sup>dim</sup>. All gates were set using respective isotype controls.</p
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
(A) For panel 2, the first gate (G1) is on time vs CD45 to exclude poor flow, the second gate (G2) i...
<p>Monocytes were first selected on the basis of their physical parameters, i.e. forward scatter (FS...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p><b>A.</b> PBMC and <b>B.</b> MBC. Initial gating is performed from the SSC-A vs. FSC-A to determi...
<p>Gating Strategy. Panel A. Identification of total monocytes from peripheral blood mononuclear cel...
<p>Monocytes were defined as CD45 positive cells (B) exhibiting a typical forward (FSC) and sideward...
<p>(A) Monocytes (CD14<sup>+</sup>), B cells (CD19<sup>+</sup>) were gated from PBMC. pDC were direc...
<p>PBMCs were gated to eliminate doublets and dead. CD3+lymphocytes were identified and gated for CD...
<p>(A) Selection of populations containing single cell CD4 or CD8 T cells. Each subsequent panel sho...
<p>Leukocytes were selected in the forward-sidescatter plot (fig. 1A) and copied to a sidescatter-CD...
<p>Monocytes were defined as CD45 and HLA-DR expressing and lineage (CD2, CD3, CD15, CD19, CD56, NKp...
<p>(A) Lymphocytes were gated according to forward and side scatter (R1), (B) CD4<sup>+</sup>CD25<su...
<p>(A) After gating for single cells in forward scatter area (FSC-A) versus height (FSC-H) and side ...
<p>The gating strategy applied for mucosal mononuclear cells (MMC) and peripheral blood mononuclear ...
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
(A) For panel 2, the first gate (G1) is on time vs CD45 to exclude poor flow, the second gate (G2) i...
<p>Monocytes were first selected on the basis of their physical parameters, i.e. forward scatter (FS...
<p>a) Viable cells were gated based on negative expression of the fixable viability stain (FVS). Tru...
<p><b>A.</b> PBMC and <b>B.</b> MBC. Initial gating is performed from the SSC-A vs. FSC-A to determi...
<p>Gating Strategy. Panel A. Identification of total monocytes from peripheral blood mononuclear cel...
<p>Monocytes were defined as CD45 positive cells (B) exhibiting a typical forward (FSC) and sideward...
<p>(A) Monocytes (CD14<sup>+</sup>), B cells (CD19<sup>+</sup>) were gated from PBMC. pDC were direc...
<p>PBMCs were gated to eliminate doublets and dead. CD3+lymphocytes were identified and gated for CD...
<p>(A) Selection of populations containing single cell CD4 or CD8 T cells. Each subsequent panel sho...
<p>Leukocytes were selected in the forward-sidescatter plot (fig. 1A) and copied to a sidescatter-CD...
<p>Monocytes were defined as CD45 and HLA-DR expressing and lineage (CD2, CD3, CD15, CD19, CD56, NKp...
<p>(A) Lymphocytes were gated according to forward and side scatter (R1), (B) CD4<sup>+</sup>CD25<su...
<p>(A) After gating for single cells in forward scatter area (FSC-A) versus height (FSC-H) and side ...
<p>The gating strategy applied for mucosal mononuclear cells (MMC) and peripheral blood mononuclear ...
<p>We identified T cells from total leukocytes as shown. We first selected singlets using forward sc...
(A) For panel 2, the first gate (G1) is on time vs CD45 to exclude poor flow, the second gate (G2) i...
<p>Monocytes were first selected on the basis of their physical parameters, i.e. forward scatter (FS...