Flow cytometry gating strategy for monocyte/DC/NK panel.
- Whitney C. Weber (8566797)
- Caralyn S. Labriola (10777135)
- Craig N. Kreklywich (7592762)
- Karina Ray (4397881)
- Nicole N. Haese (3826969)
- Takeshi F. Andoh (8299272)
- Michael Denton (5120)
- Samuel Medica (17081565)
- Magdalene M. Streblow (14784627)
- Patricia P. Smith (17420031)
- Nobuyo Mizuno (9096782)
- Nina Frias (17420034)
- Miranda B. Fisher (17420037)
- Aaron M. Barber-Axthelm (10777126)
- Kimberly Chun (17420040)
- Samantha Uttke (12436969)
- Danika Whitcomb (17420043)
- Victor DeFilippis (200525)
- Shauna Rakshe (17420046)
- Suzanne S. Fei (1278480)
- Michael K. Axthelm (8399388)
- Jeremy V. Smedley (10777138)
- Daniel N. Streblow (7592771)
Publication date
November 2023
DOIAbstract
Gating strategy for monocyte/DC/NK panel is shown. Monocytes and macrophages were defined as CD3-/CD20-/CD8-/HLA-DR+ with classical monocytes being CD16-/CD14+, intermediate monocytes being CD16+/CD14+, and non-classical monocytes being CD16+/CD14-. DCs were defined as CD3-/CD20-/CD8-/HLA-DR+/CD16-/CD14- with myeloid DCs being CD11c+/CD123- and plasmacytoid DCs being CD11c-/CD123+. Activated cells within each subset were defined as CD169+. (EPS)</p
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