An example of flow cytometric analysis of human peripheral blood sorted on CD45^dim cells in a patient with DFU (A) and a healthy control subject (B).

<p>The triple positive phenotype (CD34⁺/KDR⁺/CD133⁺) was determined by gating the CD133⁺ cells on the CD34⁺/KDR⁺. 1.000.000 events per sample were acquired and the counts for each phenotype are shown in the picture. Smaller counts in all phenotypew were observed in the diabetic patient (A) when compared to the healthy subject (B) in the double and triple measurements.