Representative flow cytometry analysis for quantifying the number of endothelial progenitor cells and endothelial microparticles.

<p>(A) The gate was set using sizing microparticles (<1.5mm). Endothelial apoptotic microparticles were defined as particles positively labeled for CD31 and annexin V (CD31⁺/annexin V⁺). (B) Mononuclear cells (MNCs) were gated by forward/sideward scatter (FSC/SSC). The numbers of circulating endothelial progenitor cells (EPCs) were gated with monocytes and defined as CD34⁺KDR⁺CD133⁺. FITC, fluorescein isothiocyanate.