<p>CD45<sup>+</sup> cells were gated and proportions of CD14<sup>+</sup>, CD3<sup>+</sup>, CD15<sup>+</sup> and CD19<sup>+</sup> cells were analyzed. The cases were divided into three groups according to CD14<sup>+</sup> and CD3<sup>+</sup> cell proportions, namely macrophage-dominated (B), T-lymphocyte-dominated (C) and mixed (D) groups. Average values (mean + SEM) of the proportions of different cell types in the three different phenotypes of inflammation (A) with representative flow cytometry blots (B-D) are shown. n = 7 in macrophage-dominated group, n = 6 in T-lymphocyte-dominated group and n = 3 in mixed group. ***p<0.001</p
<p>Cells from blood and lungs were isolated at time 0 and 48 h after CpG ODN administration, but bef...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>A total of 30 fresh surgical CRC tumor tissues were digested for characterization of tumor infilt...
<p>Peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) were obtai...
<p><b>A</b>) Representative flow cytometry plots illustrating the typical gating strategy used to id...
Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However, a metho...
<p>After 3 passages, CD45<sup>−</sup>/Ter119<sup>−</sup> cells were stained with the following surfa...
<div><p>Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However,...
<p>(<b>A</b>) The gating strategy to analyze CD45<sup>+</sup> leukocytes from a non-aneurysmal abdom...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>PBMC were obtained from blood samples and stained for CD45, NK and CD3 with fluorescent antibodie...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>Cells from blood and lungs were isolated at time 0 and 48 h after CpG ODN administration, but bef...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>A total of 30 fresh surgical CRC tumor tissues were digested for characterization of tumor infilt...
<p>Peripheral blood mononuclear cells (PBMCs) and pleural fluid mononuclear cells (PFMCs) were obtai...
<p><b>A</b>) Representative flow cytometry plots illustrating the typical gating strategy used to id...
Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However, a metho...
<p>After 3 passages, CD45<sup>−</sup>/Ter119<sup>−</sup> cells were stained with the following surfa...
<div><p>Flow cytometry is used extensively to examine immune cells in non-lymphoid tissues. However,...
<p>(<b>A</b>) The gating strategy to analyze CD45<sup>+</sup> leukocytes from a non-aneurysmal abdom...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>PBMC were obtained from blood samples and stained for CD45, NK and CD3 with fluorescent antibodie...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>Cells from blood and lungs were isolated at time 0 and 48 h after CpG ODN administration, but bef...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>A total of 30 fresh surgical CRC tumor tissues were digested for characterization of tumor infilt...