<p><b>A</b>) Representative flow cytometry plots illustrating the typical gating strategy used to identify the viable immune cells in blood and tumor. <b>B</b>) The proportion of live CD45<sup>+</sup> cells was calculated for each specimen. <b>C</b>) CD3<sup>+</sup> T cells consistently comprised the majority of the viable immune cells in blood and tumor, while CD19<sup>+</sup> B cells and non-T or B cells comprise smaller proportions.</p
<p>Flow cytometry staining was performed for T cells on blood and lymphoid organs (spleen, bone marr...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International I...
<p>CD45<sup>+</sup> cells were gated and proportions of CD14<sup>+</sup>, CD3<sup>+</sup>, CD15<sup>...
All flow cytometry data are represented as percent total live cells, which include tumor cells and i...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
A, Percentages of myeloid and TAM cell populations. Upper panel: TAMs, pDCs, granulocytes, and monoc...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>T-cell subsets were based on the CD27 and CD45RO cell surface markers. A HAT patient before and a...
<p>PBMC were analyzed by flow cytometry. (<b>A</b>) Representative plots gated on live, CD14-CD3-CD4...
Representative plots showing activated B cells (ABC) as CD19+CD10-IgD-CD71+CD38-CD20+ and antibody s...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
<p>Flow cytometry staining was performed for T cells on blood and lymphoid organs (spleen, bone marr...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International I...
<p>CD45<sup>+</sup> cells were gated and proportions of CD14<sup>+</sup>, CD3<sup>+</sup>, CD15<sup>...
All flow cytometry data are represented as percent total live cells, which include tumor cells and i...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
A, Percentages of myeloid and TAM cell populations. Upper panel: TAMs, pDCs, granulocytes, and monoc...
<p>(A) The analysis of immune cell subsets from peripheral blood. Lymphocytes were first gated from ...
<p>T-cell subsets were based on the CD27 and CD45RO cell surface markers. A HAT patient before and a...
<p>PBMC were analyzed by flow cytometry. (<b>A</b>) Representative plots gated on live, CD14-CD3-CD4...
Representative plots showing activated B cells (ABC) as CD19+CD10-IgD-CD71+CD38-CD20+ and antibody s...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
<p>Flow cytometry staining was performed for T cells on blood and lymphoid organs (spleen, bone marr...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International I...