Flow cytometry analysis of key immune cell populations in 100mm³ tumors stratifies each syngeneic model by immune cell content.

All flow cytometry data are represented as percent total live cells, which include tumor cells and immune infiltrates. (A) Pie charts summarizing the median abundance (% total live cells) of eight different immune populations in 100mm3 tumors from each syngeneic model. Percent values in pie charts refer to “other cells,” which includes tumor and other immune cells not captured in this analysis. Plots showing abundance of (B) T cell populations, (C) NK and B cell populations, and (D) myeloid cell populations in 100mm3 tumors. Medians of each immune population are indicated as bars and these values were used in the pie charts shown in (A). Statistical significance between groups: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001.