Appressorium formation in <i>coira1</i> mutants of <i>C. orbiculare</i> on glass slides.

<p>(A) Conidial suspensions of each strain in distilled water were incubated on multiwell glass slides at 24°C for 24 h. WT, the wild-type 104-T; DL1-1 and DL1-2, the <i>coira1</i> mutants; CL1-1, the <i>CoIRA1</i>-complemented transformant of DL1-1; CL1-2, the <i>CoIRA1</i>-complemented transformant of DL1-2; EL1-1, ectopic strain. Scale bar, 10 µm. (B) Percentages of conidial germination, appressorium formation, and abnormal appressorium formation in the <i>C. orbiculare</i> WT and <i>coira1</i> mutants on multiwell glass slides. Approximately 100 conidia of each strain were observed per well on multiwell slide glass. Three replicates were examined. Three independent experiments were conducted, and standard errors are shown. Black bar, conidial germination; gray bar, appressorium formation; white bar, abnormal appressorium formation.