Growth, sporulation, germination and appressorium formation by the wild-type (WT), ectopic (Ect) and mutant strains of <i>ΔcrzA</i> (Δ<i>crzA31A</i>, Δ<i>crzA36A</i> and Ect<i>crzA37B</i>) and <i>Δslt</i>A (Δ<i>sltA10A</i>, Δ<i>sltA21C</i> and Ect<i>sltA1C</i>) in glucose minimal media amended (□), or not (■), with KCl.

<p>These <i>C</i>. <i>gloeosporioides</i> strains were disc-inoculated on glucose minimal media amended with 25 mM KCl and incubated for 5 days at 24°C. (A, E) Radial colony growth, (B, F) sporulation, (C, G) germination and (D, H) appressorium formation were evaluated after 16 h of incubation on glass slides at 24°C. Experiments were repeated three times and results of a single representative experiment are shown. Columns with different letters (lower or upper case) are significantly different at P ≤ 0.05 according to the Tukey-Kramer multiple comparison test.