Appressorium formation assay of DRS2/DAMK1 and DMK1/DARS2 on the glass slides.

<p>(A) Conidial suspensions of each strain in distilled water incubated on multiwell glass slides at 24°C for 24 h. DRS2, the <i>coras2</i> mutant; DMK1, the <i>comekk1</i> mutant; DRS2/DAMK1, DRS2 transformed with a dominant active form <i>CoMEKK1</i>; DMK1/DARS2, DMK1 transformed with a dominant active form <i>CoRAS2</i>. Scale bar, 10 µm. (B) Percentages of conidial germination and appressorium formation in <i>C. orbiculare</i> on multiwell glass slides. Approximately 100 conidia of each strain were observed per well on the multiwell slide glass. Three replicates were examined. Three independent experiments were conducted, and standard errors are shown. Black bar, conidial germination; gray bar, appressorium formation.