<i>agef-1(vh4</i>) suppresses the <i>lin-2(e1309)</i> Vul phenotype, has large vesicles in coelomocytes and a dumpy body morphology.

<p>(A–D) Representative Differential Interference Contrast (DIC) images of vulvas of wild-type, <i>lin-2(e1309)</i>, <i>agef-1(vh4)</i> and <i>agef-1(vh4); lin-2(e1309)</i> L4 stage larvae. The <i>lin-2(e1309)</i> larva lacks a vulva while <i>agef-1(vh4); lin-2(e1309)</i> has a second vulval invagination and hence is Muv. <i>agef-1(vh4)</i> single mutants have a wild-type vulva. Bar, 10 µm. (E, F) DIC images of coelomocyte pairs (white arrows) of wild-type and <i>agef-1(vh4)</i> L4 larvae. <i>agef-1(vh4)</i> coelomocytes have enlarge vesicles as compared to wild-type. Bar, 10 µm. (G, H) DIC images of adult wild-type and <i>agef-1(vh4)</i> animals; <i>agef-1(vh4)</i> mutants have a smaller body length compared to wild-type. Bar, 100 µm.