vulF cells, LIN-11, and histone demethylases are required to activate <i>unc-4</i> expression in vulval VC neurons.

<p>(A) Adult expression of <i>uIs45</i> in <i>syIs66[B0034.1::pes-10::GFP]</i> animals with vulF cells ablated at the L4 stage. The absence of <i>syIs66</i>, which labels vulF cells, confirmed their successful ablation. The asterisk indicates the position of the vulva. Scale bar = 100 µm. (B) Expression of <i>uIs45</i> and VC marker <i>vsIs13</i> in <i>lin-11(n389)</i> adults. (C) Effect of expression of <i>lin-11(+)</i> driven by an VC-specific promoter on <i>uIs45</i> expression in <i>lin-11(n389); uIs45</i> animals. The vulval VC neurons are indicated by arrows. (D) Expression of <i>syIs80[lin-11p::GFP]</i> in VC neurons in adults (left) and L2 larvae (right). (E) Adult expression of <i>uIs45</i> in wild-type, <i>jmjd-2(tm2966)</i>, and <i>spr-5(by134)</i> animals. Arrows indicate the positions of VC4 and VC5.