AGEF-1 and the AP-1 complex regulate the size of late endosomes/lysosomes in coelomocytes.

<p>(A, B) Confocal images of the coelomocytes of wild-type and <i>agef-1(vh4)</i> L4 larvae expressing the early endosomal marker 2×FYVE::GFP. (C) Quantification of the diameter of the largest 2×FYVE::GFP-positive vesicle per coelomocyte in wild-type and <i>agef-1(vh4)</i>. (D, E) Confocal images of the coelomocytes of wild-type and <i>agef-1(vh4)</i> L4 larvae expressing the late endosomal/lysosomal marker LMP-1::GFP. (F) Quantification of the diameter of the largest LMP-1::GFP-positive vesicle per coelomocyte in wild-type and <i>agef-1(vh4)</i>. (G, H) Epifluorescent images of the coelomocytes of <i>apm-1(RNAi) unc-101(sy108)</i> and <i>unc-101(sy108) apg-1(RNAi)</i> L4 larvae. (I) Quantification of the diameter of the largest LMP-1::GFP-positive vesicle per coelomocyte upon depletion of multiple AP-1 subunits. Prism 5 (GraphPad Software, Inc., La Jolla, CA) was used for statistical analysis; unpaired t-test was performed to compare changes in the vesicle size. *** <i>P<0.001</i>. Shown is the mean vesicle size plus standard error of the mean. All bars, 5 µm.