Increased CD44^hi CD8⁺ T cell subsets in <i>Hdac1^f/fCd4Cre</i> mice.

<p>(A) Plots depict CD44 and CD62L expression on splenic <i>Hdac1^f/f</i> and <i>Hdac1^f/fCd4Cre</i> CD8⁺ T cells. Data shown are representative of 6 mice per genotype, analyzed in 4 independent experiments. (B) Diagrams showing the summary of the percentage of CD44^l°CD62L⁺, CD44^hiCD62L⁺ and CD44^hiCD62L^– CD8⁺ T cells (mean ± SEM; n = 6). (C) <i>Hdac1^f/f</i> and <i>Hdac1^f/fCd4Cre</i> splenocytes were stimulated <i>ex vivo</i> with PMA/ionomycin for 4 h and IFNγ production was analyzed by intracellular cytokine staining. Plots show IFNγ versus CD44 expression on gated CD8⁺ T cells. Data are representative of 5 mice analyzed in 3 independent experiments. (D) Summary of all PMA/ionomycin stimulation experiments as described in C. The left diagram depicts the percentage of IFNγ-producing CD8⁺ T cells, while the diagram to the right indicates the percentage of IFNγ-producing cells within the CD44^hiCD8⁺ T cell population. Mean ± SEM is shown (n = 5). (C, D) Numbers in the dot plot indicate the percentage of cells in the respective quadrants. (B, D) Statistical analysis was performed by using a two-tailed non-paired Student’s t test. The P-values were defined as following: *, P<0.05; **, P<0.01; ***, P<0.001.