Multifunctional CD4⁺ cells have a CD44^hiCD62L^loCD27^−ve phenotype.

<p>Spleen cells isolated from six week BCG immunized mice were stained for cytokine production by ICS and phenotyped for CD44, CD62L and CD27 expression. Regions represent % of cells exhibiting a CD4⁺CD44^hiCD62L^lo (A) or CD4⁺CD44^hiCD27⁻ (b) phenotype. Plots are gated on CD4⁺ cells and are representative of 1 of 3 independent experiments.