Increased effector/memory CD4⁺ T cells in T-TRAF3^−/− mice.

<p>Splenocytes were stained for CD4, Foxp3, CD44 and CD62L. CD4⁺Foxp3⁻ T cells were gated for CD44 and CD62L analysis. (<b>A</b>) percentages, (<b>B</b>) numbers of naïve and (<b>C</b>) numbers of CD44^hiCD62L^l°^w effector/memory T cells are shown. Data represent 10 mice in each group. (<b>D</b>) and (<b>E</b>) Splenocytes were stimulated with PMA and ionomycin for 6 hr. Intracellular staining for cytokine production in CD4⁺ T cell subsets was performed. (<b>E</b>) Summarized data from three individual experiments. *p<0.01.