Naïve CD8⁺ but not CD4⁺ T cells were significantly decreased in the periphery of Tsc1 KO mice.

<p>The peripheral T cell subsets of WT and Tsc1 KO mice were detected using FCM 4–5 wks after birth. <b>A.</b> Total thymic cell number and thymocyte subset profile of Tsc1 KO mice were identical as WT littermates. <b>B.</b> Total cell number of spleen, pLNs and mLNs of WT and Tsc1 KO mice. <b>C.</b> The percentage of CD8⁺T but not CD4⁺T cells decreased dramatically in all peripheral lymphoid organs of Tsc1 KO mice. <b>D.</b> The FACS profile analysis of CD62L, CD45RB and CD44 on pLN CD4⁺ T cells was evaluated. The frequency of naïve CD62L^hi, CD45RB^hi and CD44^low CD4⁺T cells in spleen, pLN and mLN of Tsc1 KO mice was summarized. <b>E.</b> The FACS profile analysis of CD62L, CD45RB and CD44 on the gated pLN CD8⁺ T cells was evaluated. The frequency of naïve CD62L^hi, CD45RB^hi and CD44^low CD8⁺T cells in spleen, pLN and mLN of Tsc1 KO mice was summarized. *p<0.05; **p<0.01; ***p<0.001. Data were shown as Mean±SD (N = 6).