Tumor necrosis factor-α (TNFα)-mediated hepatocyte death is suppressed by pretreatment with Neurotropin (NTP).

<p><b>(A-D)</b> Wild type (WT) primary hepatocytes were pretreated with 0.2 NU/mL NTP for 1 hour followed by the treatment with 2 ng/mL TNFα with or without 200 ng/mL Actinomycin D for 16 (A, B) 8 (C) or 4 hours (D). Representative TUNEL staining (A; Red, TUNEL staining; Blue, Nucleus) and quantification (B) are shown. Data represent the mean±SEM of triplicate cultures. Western blots for caspase-3, cleaved caspase-3 (C), phospho-JNK, total JNK and β-actin are shown (D). Representative blots are shown. Similar results were obtained in three independent experiments.